Telomerase Repeated Amplification Protocol (TRAP)
نویسندگان
چکیده
منابع مشابه
Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP)
The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomeras...
متن کاملTelomerase activity in the various regions of mouse brain: non-radioactive telomerase repeat amplification protocol (TRAP) assay.
Telomerase, a ribonucleoprotein, is responsible for maintaining the telomere length and therefore promoting genomic integrity, proliferation, and lifespan. In addition, telomerase protects the mitochondria from oxidative stress and confers resistance to apoptosis, suggesting its possible importance for the surviving of non-mitotic, highly active cells such as neurons. We previously demonstrated...
متن کاملEvaluation of the Telomeric Repeat Amplification Protocol (TRAP) Assay for Telomerase as a Diagnostic Modality in Recurrent Bladder Cancer1
There is a need for the identification of an accurate test for the detection of recurrent bladder cancer. In this study, we evaluate the telomeric repeat amplification protocol (TRAP) assay for detection of tebomerase as a potential new method for bladder cancer detection and compare it to voided urine cytology. A urine sample and a bladder wash were obtained from 63 patients with a history of ...
متن کاملSimplified method for the telomeric repeat amplification protocol (TRAP).
Since it was first described by Kim et al. in 1994 (4), the telomeric repeat amplification protocol (TRAP) has been used extensively to detect telomerase activity in a variety of tissues. It is a highly sensitive polymerase chain reaction (PCR)-based assay that has been used to detect telomerase activity in a high proportion of cancers of various origins as well as low levels of activity in cer...
متن کاملQuantitative telomerase activity in circulating human leukocytes: utility of real-time telomeric repeats amplification protocol (RQ-TRAP) in a clinical/epidemiological setting.
BACKGROUND There is accumulating evidence from the epidemiological field of telomere biology that telomere length plays an important role in the pathophysiology of cardiovascular disease. The RNA-dependent DNA polymerase, telomerase, is essential in regulating telomere length by acting as a reverse transcriptase. However, the relationship between telomerase activity and telomere length in cardi...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2015
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1657